Figure 3
Effects of the treatment of the agonistic anti-Fas antibody or etoposide on the apoptotic execution of HeLa.S-Fucci cells. Cells were cultured in the presence of 0.5 μg/ml of the agonistic anti-Fas antibody (a) or 50 μM etoposide (VP-16) (b) for 24 h, and were observed with fluorescence microscope. The morphology of the cells and the color of cell nuclei at 6 h for Fas-treated cells (a) and at 24 h for etoposide-treated cells (b) were shown by phase contrast, mKO2, mAG1, and merged images, respectively. Apoptotic cells with mKO2 were marked with red arrows, apoptotic cells with both mKO2 and mAG1 were with yellow arrows, apoptotic cells with mAG1 were with green arrows, and apoptotic cells without any fluorescent proteins were with white arrows
