Figure 5
From: Intrinsic regulation of hemangioma involution by platelet-derived growth factor
PDGF-BB inhibits adipogenic differentiation in hemSCs. (a) Representative oil red O staining of hemSCs cultured in adipogenic differentiation media supplemented with 10 ng/ml of PDGF-AA, -AB, or -BB. (b) Quantitative analysis of adipogenic differentiation in the presence of PDGF polypeptides was assessed by real-time RT-PCR for adipogenesis-specific transcription factors, C/EBPα and PPARγ (data normalized to 18 S rRNA and presented as relative to adipogenic media; *P<0.05 compared with the adipogenic media; n=3). (c) Effect of adipogenic differentiation on PDGFR expression in hemSCs at day 7. (d) Oil red O staining of hemSCs cultured with different growth factors (each at 10 ng/ml) illustrating specific inhibition of hemSC adipogenesis by PDGF signaling
