Figure 4 | Cell Death & Disease

Figure 4

From: Functional interplay between p63 and p53 controls RUNX1 function in the transition from proliferation to differentiation in human keratinocytes

Figure 4

p53 cooperates with ΔNp63 to regulate RUNX1 in HPK. (a) RUNX1 transcript levels was quantified by RT-QPCR after treatment of proliferative or differentiated HPK with control or p53 siRNA. The effect of p53 siRNA on p53 expression was shown in Figure 2b. (b) p53, ΔNp63 and RUNX1 transcript levels were quantified by RT-QPCR after treatment of proliferative (left panel) or differentiated (right panel) HPK with control or p53 and ΔNp63 siRNA. (c) RUNX1 protein level was assessed by WB 48 h after treatment of proliferative (upper panel) or differentiated (lower panel) HPK with both p53 and ΔNp63 siRNA. (d) ΔNp63 (left panel) and RUNX1 (right panel) transcripts levels were quantified by RT-QPCR after treatment of proliferative or differentiated HaCaT cells with control or ΔNp63 siRNA. (e) RUNX1 transcripts were measured by QPCR at different stages of the HaCaT cell cultures (left panel). Involucrin (INV) and RUNX1 proteins expression was assessed by immunoblotting at different stages of the HaCaT culture (right panel)

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