Figure 6 | Cell Death & Disease

Figure 6

From: Functional interplay between p63 and p53 controls RUNX1 function in the transition from proliferation to differentiation in human keratinocytes

Figure 6

RUNX1 represses human keratinocyte proliferation. (a) RUNX1 overexpression using pCMV-RUNX1 was verified by western blotting (left panel) compared with an empty vector and cell numbers (right panel) were determined. Error bars are mean +/−S.D. of triplicate samples from one representative experiment. (b) RUNX1 mRNA (upper left panel) and protein (lower left panel) levels were measured after RUNX1 silencing by an siRNA targeting RUNX1 versus a control non-targeting siRNA in proliferating keratinocytes and cell numbers were determined (right panel). (c) Proliferation study using BrdU incorporation assay was performed in triplicate in three independent experiments with different donors. (d) Cell cycle analysis of siRUNX1-transfected keratinocytes shows percentages of cell cycle phases (R1=G0/G1, R2=S, R3=G2/M, left panel). Dot plots illustrate one representative experiment. The graph (right panel) shows the percentage of stained RUNX1 siRNA-treated keratinocytes/ the percentage of stained control siRNA-treated keratinocytes. (e) p21, PCNA and Ki67 transcripts levels were quantified by RT-QPCR 24 h after treatment of proliferating primary keratinocytes with control or RUNX1 siRNA

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