Figure 5

Inflammation activates the ER apoptotic pathway in OPCs, an effect that was attenuated by CBD. (a) CBD attenuated the inflammation-induced increase in CHOP expression. (b) The upregulation of caspase 12 expression in conditions of inflammation was attenuated by CBD. (c) CBD restores the Bcl-2/Bax balance in OPCs treated with LPS/IFNγ. (d) CBD increases the expression of the negative feedback regulator GADD34 under conditions of inflammation. In all cases, OPCs were incubated with LPS/IFNγ in the presence or absence of CBD (1 μM). The mRNA expression of each gene was measured 24 h later by quantitative RT-PCR and normalized to the expression of the 18S gene. The data represent the mean±S.E.M. of three independent cultures analyzed in triplicate, and the statistical significance was determined using Kruskal–Wallis ANOVA followed by Mann–Whitney U test: *P<0.05, ***P⩽0.001 versus untreated cells; and ^#P⩽0.05, ^##P⩽0.01 versus cells exposed to LPS/IFNγ alone