Figure 1 | Cell Death & Disease

Figure 1

From: HtrA2 deficiency causes mitochondrial uncoupling through the F1F0-ATP synthase and consequent ATP depletion

Figure 1

Mitochondria are depolarised in HtrA2-deficient cells. (a) Mitochondrial membrane potiential was estimated by live cell imaging in a range of primary cell types using TMRM in redistribution mode (25 nM). Data are normalised to midbrain astrocytes (100%) and are represented as mean±S.E.M. (b) Mitochondrial hyperpolarisation (ΔTMRM) in response to oligomycin (2 μg/ml) is presented as a percentage of the difference between the initial TMRM fluorescence and the minimum TMRM fluorescence achieved after addition of FCCP (1 μM; see Figure 1c). (c and d) Representative TMRM traces for one WT (c) and one KO (d) midbrain neuron, showing responses to oligomycin (2 μg/ml), rotenone (5 μM) and FCCP (1 μM). In all cases, * indicates P<0.05 and ** indicates P<0.01 compared with WT values

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