Figure 3 | Cell Death & Disease

Figure 3

From: HtrA2 deficiency causes mitochondrial uncoupling through the F1F0-ATP synthase and consequent ATP depletion

Figure 3

Oxygen consumption is increased in HtrA2-deficient cells. (a) Oxygen consumption was measured in whole immortalised MEFs using a Clark oxygen electrode. Respiration was inhibited by blocking ATP production using oligomycin (2 μg/ml), and maximised by adding the uncoupler FCCP (1 μM). (b and c) Whole mitochondria were isolated from the brains of WT and HtrA2-KO mice and oxygen consumption was measured in state III (in which ADP is provided and respiration is coupled to oxidative phosphorylation), (b) and state IV (in which no ADP is present), (c). The experiment was performed in the presence of substrates for complex I (5 mM glutamate and 5 mM malate) or substrates for complex II (5 mM succinate in the presence of 5 μM rotenone). (d) RCR (ratio of state III to state IV respiration) was significantly reduced in mitochondria from the brains of HtrA2-KO mice compared with WT. Data is represented as mean±S.E.M. In all cases, * indicates P<0.05 and ** indicates P<0.01 compared with WT values

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