Figure 4 | Cell Death & Disease

Figure 4

From: HtrA2 deficiency causes mitochondrial uncoupling through the F1F0-ATP synthase and consequent ATP depletion

Figure 4

Mitochondrial ROS production is decreased in HtrA2-deficient cells. WT and HtrA2-KO midbrain cultures were loaded with MitoSOX (5 μM), an indicator of mitochondrial ROS production, and monitored by fluorescence microscopy. The rate of increase in MitoSOX fluorescence (indicating mROS production) was significantly reduced in HtrA2-KO cells compared with WT. Neurons of both genotypes responded equally to stimulation of ROS production using rotenone (5 μM). Data are represented as mean±S.E.M. ** Indicates P<0.05

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