Figure 5 | Cell Death & Disease

Figure 5

From: Primary hematopoietic cells from DBA patients with mutations in RPL11 and RPS19 genes exhibit distinct erythroid phenotype in vitro

Figure 5

Delayed erythroid differentiation following depletion of RPL11. (a) Decreased numbers of CD36+/GPA+ cells following infection with shRPL11 compared with shRPS19-infected and various control cells (three independent experiments). (b) Less CD71+ cells and more immature CD34+ cells following infection with shRPL11 compared with shRPS19-infected and various control cells (three independent experiments). (c) Top and bottom panels: increased apoptosis following depletion of RPL11, with more Annexin-V-positive cells in cells infected with shRPL11 compare with RPS19 depletion (three independent experiments). (d) Expression level of the major proteins involved in apoptosis (caspase 3 and cleaved caspase 3, PARP and cleaved PARP) or anti-apoptotic protein such as Bcl-XL following of infection of cord blood CD34+ with specific shRPS19 or shRPL11 compared with the shSCR infection

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