Figure 5
Administration of TZD or HFD drives opposing effects on mouse prostate metabolism and differentiation in vivo. (a) Western diet-, but not TZD-fed animals significantly increased total weights by 20 g (N=3 each). (b) PPARγ2 is coexpressed in prostate smooth muscle. (c–n) C57B male mice were fed control (a, f, i and l), Rosiglitazone (b, g, j and m) or Western diet chow (e, h, k and n) for 6 months. PPARγ2 levels were similar in prostate epithelia after TZD treatment (f versus g) but decreased in some acini of HFD-treated animals (h, star). Intramuscular adipocytes were increased in TZD-treated animals (g, star), which also resulted in increased AR expression increased in smooth muscle (j, arrow). AR expression was decreased in HFD-treated animals (* in k) similar to qRT-PCR analysis (see Table 2). PDK4 expression was similar in prostate tissue of TZD-treated animals (m, * indicates adipose) and decreased in HFD-treated animals (n) (see Table 2)
