Table 1 Comparison of gene expression from mPrE-PPARγKO+EV, +PPARγ1 and +PPARγ2-restored cell lines by qRT-PCR

From: PPARγ isoforms differentially regulate metabolic networks to mediate mouse prostatic epithelial differentiation

   

+PPARγ1

+PPARγ2

Gene

Function

Cellular localization

Fold induction

P value

Fold induction

P value

I. Metabolic genes

 Acot1

Long chain fatty acid metabolism

Cytoplasm

19.0

0.09

304.3

<0.05

 Acsf2

Fatty acid oxidation

Mitochondrion

69.1

<0.05

109.7

0.10

 Adipor1

Fatty acid oxidation

Plasma membrane

1.7

<0.05

2.7

<0.05

 Cd68

Fatty acid transport

Lysosome

2.1

0.27

11.8

<0.05

 Cd36

Long chain fatty acid metabolism

Cytoplasm, mitochondrion

3.2

0.09

32.1

<0.05

 Dagla

Lipolysis

Plasma membrane

3.5

<0.05

5.5

0.08

 Dgat2

Triglyceride synthesis

Endoplasmic reticulum

1.4

0.10

3.3

0.08

 Elovl4

Fatty acid elongation

Endoplasmic reticulum

−2.6

0.09

1.3

0.41

 Fabp4

Fatty acid transport

Cytoplasm, nucleus

72.7

<0.05

220.3

<0.05

 Fbp2

Carbohydrate metabolism

Cytoplasm

−3.7

<0.05

2.1

0.11

 Fetub

Insulin responsiveness

Extracellular

3.2

0.30

2.4

0.13

 Gls2

Glutamine synthesis

Mitochondrion

−1.3

0.52

−1.2

0.48

 Glul

Glutamine catabolism

Mitochondrion

1.7

0.25

2.9

0.16

 Lipa

Lipolysis

Lysosome

3.7

<0.05

6.1

<0.05

 Lpl

Lipolysis

Plasma membrane

15.4

<0.05

121.1

<0.05

 Lrp1

Fatty acid transport

Plasma membrane

2.1

0.10

8.3

<0.05

 Pdk4

Carbohydrate metabolism

Mitochondrion

17.4

<0.05

5.1

<0.05

 Pparg

Nuclear receptor

Nucleus

8.7

0.06

64.6

<0.05

 Ppargc1a

Pparg cofactor

Nucleus

6.0

0.09

23.9

<0.05

 Ppargc1b

Pparg cofactor

Nucleus

1.5

0.30

2.2

0.19

 Scd1

Fatty acid desaturase

Endoplasmic reticulum

−2.2

0.07

2.6

<0.05

 Txnip

Carbohydrate metabolism

Mitochondrion

1.1

0.64

7.9

0.08

II. Oxidative stress genes

 Aldh1a1

Oxidative stress

Cytoplasm

1.3

0.28

9.2

0.06

 Aldh1a7

Oxidative stress

Cytoplasm

2.0

0.12

8.7

<0.05

 Aldh3a1

Oxidative stress

Cytoplasm

4.8

0.19

11.8

<0.05

 Aldh3b1

Oxidative stress

Cytoplasm

3.7

0.16

9.7

<0.05

 Aldh7a1

Oxidative stress

Cytoplasm

4.9

<0.05

2.9

0.12

 Aox3

Oxidative stress

Cytoplasm

105.9

0.20

92.6

<0.05

 Casp4

Cell death

Endoplasmic reticulum

5.0

<0.05

21.6

<0.05

 Cat

Oxidative stress

Mitochondrion

1.2

0.36

4.1

<0.05

 Cyp17a1

Oxidative stress

Mitochondrion

3.5

<0.05

4.5

0.09

 Dhrs3

Oxidative stress

Plasma membrane

13.8

0.23

31.9

<0.05

 Gsta2

Oxidative stress

Cytoplasm

3.9

0.07

18.7

0.24

 Sirt5

Oxidative stress

Mitochondrion

1.9

0.13

2.8

0.08

 Sod3

Oxidative stress

Extracellular

5.1

<0.05

8.1

0.11

III. Differentiation genes

 Ar

Steroid receptor

Cytoplasm, nucleus

3.5

<0.05

10.8

<0.05

 Krt14

Basal cell keratin

Plasma membrane

−1.2

0.60

4.1

0.14

 Pbsn

Prostate-specific differentiation

Extracellular

−1.7

0.41

2.7

0.19

 Pten

Lipid and protein phosphatase

Plasma membrane

1.5

0.14

2.7

<0.05

 Trp63

Basal cell marker

Nucleus

3.3

<0.05

8.0

0.17

 Acta2

Smooth muscle marker

Structural

1.5

0.28

1.9

<0.05

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