Figure 6
From: FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential
FKBP51 binds to ABCG2 promoter. (a) Enriched DNA amplification bands in FKBP51-immunoprecipitated samples compared with IgG samples. ChIP with ZNF 224 served as positive control. ChIP was conducted with crosslinked chromatin, and DNA enrichment was evaluated using primers recognizing DNA-ligated poly-linkers (see Materials and Methods). G3PDH was used as ChIP quality control. (b) Enrichment of DNA regions at −3450 (blue square), −3219 (pink square), and −939 (red square) from TSS of ABCG2 gene in FKBP51-immunoprecipitated chromatin. Specificity of FKBP51 binding to ABCG2 promoter is suggested by lack of enrichment of DNA regions at −2897, −2060, and −127
