Figure 4
From: A DR6/p75NTR complex is responsible for β-amyloid-induced cortical neuron death
Anti-DR6 antibodies that block the interaction between DR6 and p75NTR inhibit Aβ42-induced neuronal cell death. (a) Anti-DR6 antibody 5D10 blocked DR6 and p75NTR complex formation from DR6/p75NTR co-transfected HEK 293 cells. Anti-DR6 antibody 2A9, which does not bind to DR6 at the p75NTR-binding site, did not block p75NTR from co-immunoprecipitating with DR6. (b) Top: Schematic domain structure of human DR6 protein depicting four extracellular CRD 1-4, a transmembrane domain (TM), and a cytoplasmic death domain (DD). Bottom: ELISA mapping 5D10-binding epitope within cysteine-rich domains of DR6 protein. (c) 5D10 blocks AP-DR6 binding to HEK 293 cells expressing p75NTR. Scale bar=25 μm. (d) Quantitative assessment of 5D10 blocking AP-DR6 binding to p75NTR from c. (e) TUNEL staining quantification of percent apoptotic cells from anti-DR6 antibody-treated and control-treated cortical neurons following Aβ42 exposure. (f) Western blot analysis of cleaved casp3 levels in anti-DR6 antibody-treated and control-treated cortical neurons following Aβ42 exposure. (g) Quantification of cleaved casp3 levels from f
