Table 1 Quantitative real-time PCR to evaluate the relative transcript levels of a panel of genes associated with glucose metabolism in cells treated with NCS or TNFα or both (Fold Change over control)

From: ATM-NFκB axis-driven TIGAR regulates sensitivity of glioma cells to radiomimetics in the presence of TNFα

Gene

TNF α

NCS

NCS+ TNF α

ACLY

−1.6947

−2.4351

−3.7477

ACO1

2.7207

−1.6575

1.0417

AGL

−1.4349

−2.856

−4.0869

ALDOB

1.2092

10.5268

9.8423

ALDOC

−2.1302

−2.6372

−4.7932

FBP1

1.2346

118.2749

124.8455

FBP2

−1.1065

17.0417

14.3105

FH

1.0785

−2.856

−2.16

G6PC

8.598

42.9921

33.8011

GALM

−1.4651

−2.2799

−4.4414

GBE1

1.0381

−5.0595

−5.5443

GCK

−1.1942

2.7435

2.7019

GYS1

2.0195

−2.0477

−1.6426

GYS2

1.2346

7.7866

7.4591

HK2

1.0636

−2.7972

−3.7218

HK3

2.5829

10.8981

6.7225

IDH1

−1.455

−1.8455

−3.0126

IDH2

1.3938

−3.147

−3.1296

PC

1.6403

−1.5199

−2.2361

PCK1

3.7685

30.0856

18.1765

PDK1

1.0028

−6.653

−5.7002

PDK2

−1.8289

−1.4035

−3.6075

PDK3

1.0028

−4.8366

−6.1946

PDK4

−4.9795

−1.1599

−2.1376

PGK1

−1.5433

−3.0504

−3.1844

PGK2

1.8013

3.62

2.0691

PGM3

−1.2535

−3.061

−2.8999

PHKA1

−6.8258

−2.6463

−4.6622

PHKB

−1.2932

−3.9014

−5.0315

PHKG1

−2.145

−2.0835

−4.5347

PRPS1L1

2.5298

35.0417

31.5376

PYGM

2.4099

17.2198

16.156

SUCLG2

−1.5757

−8.3629

−9.4873

UGP2

1.2825

−2.9059

−1.9132

  1. Abbreviation: NCS, neocarzinostatin; TNFα, tumor necrosis factor-α.
  2. Gene expression profiling of mRNA isolated from U87MG-glioma cells treated with TNFα in the presence and absence of NCS, was analyzed by qRT-PCR for genes involved in glucose metabolism. Expressions of several genes affected by the treatments are shown. Table represents the average data from two independent experiments.
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