Figure 1

Aβ induces hTau-dependent neurotoxicity and tau-independent spine loss. (a) Quantification of dead cell fluorescence intensities of hTau-expressing hippocampal slice cultures from arcAβ tg and non-tg mice treated with Aβ antibody 6E10 determined by Live/Dead cell viability/cytotoxicity assay. (b) Cytotoxicity of hTau in arcAβ tg and non-tg control slice cultures treated with 1 μg/ml of Aβ antibody 6E10, a mid-domain Aβ antibody or control antibody measured by Cytotox-Glo assay. (c) Confocal images of apical dendritic segments from CA1 neurons of arcAβ tg and non-tg slices in the presence or absence of Aβ antibody 6E10, mid-domain Aβ antibody or control antibody. (d) Quantification of spine density in cultures from arcAβ tg and non-tg mice. (e) Quantitative bar graphs representing mean values of the amount of Aβ40 and Aβ42 peptides in medium of arcAβ hippocampal slice cultures after treatment with respective Aβ antibodies as determined by ELISA. (f) Representative images of apical dendritic segments from CA1 neurons from tau^−/− mice treated with 1 μM recombinant Aβ42. (g) Quantification of spine density in tau^−/− cultures treated with recombinant Aβ42. rec. Aβ, recombinant Aβ42; mid. Ab, mid-domain Aβ antibody; contr. Ab, control antibody; mean±S.E.M.; **P<0.01 and ***P<0.001; Mann–Whitney-U-test; n=9–13, n=4 (e) scale bar: 5 μm