Figure 5 | Cell Death & Disease

Figure 5

From: NMDA receptor subunit composition determines beta-amyloid-induced neurodegeneration and synaptic loss

Figure 5

Aβ-induced caspase-3 activation causes dendritic spine loss but not hTau-dependent toxicity. (a) Western blot with antibodies against procaspase-3 and cleaved (activated) caspase-3 in slice cultures from arcAβ tg mice treated with 3 μM Ifenprodil. (b) Western blot with antibodies against procaspase-3 and cleaved caspase-3 in cultured slices from NR2AKO and NR2AKO × arcAβ tg mice. (c) Cytotoxicity of hTau in arcAβ tg and non-tg control slice cultures treated with 10 μM Z-DEVD-FMK measured by Cytotox-Glo assay. (d) Representative confocal images of apical dendritic segments from CA1 neurons of arcAβ tg and non-tg slices treated with 10 μM Z-DEVD-FMK. (e) Quantification of spine density after treatment with Z-DEVD-FMK. Z-DEVD, Z-DEVD-FMK; values are shown as mean±S.E.M. with **P<0.01 and ***P<0.001; Mann–Whitney-U-test; n=3–4 (a, b), n=7–11 (c, e); scale bar: 5 μm

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