Figure 6 | Cell Death & Disease

Figure 6

From: Oroxylin A induces dissociation of hexokinase II from the mitochondria and inhibits glycolysis by SIRT3-mediated deacetylation of cyclophilin D in breast carcinoma

Figure 6

Oroxylin A-induced SIRT3-mediated deacetylation and inhibited the peptidyl-prolyl cis-trans isomerase activity of cyclophilin D, preventing the binding of cyclophilin D to ANT. (a–c) MDA-MB-231 and MCF-7 cells were treated with 200 μ M Oroxylin A and 10 Mm NAM, respectively, or both. (a) Mitochondria were isolated and acetylated cyclophilin D (acetylated Cyp-D) was immunoprecipitated using cyclophilin D antibody. Western blot assays were performed for acetylated-lysine and cyclophilin D(Cyp-D). (b) Mitochondria were isolated and ANT-1 was immunoprecipitated using cyclophilin D antibody. Western blot assays were performed for ANT-1 and cyclophilin D (Cyp-D). (c) Cyclophilin D was immunoprecipitated from mitochondrial lysates and its peptidyl-prolyl cis-trans isomerase activity determined. Bars, S.D.(±) (d, e) MDA-MB-231 and MCF-7 cells were transfected with siRNA targeting SIRT3 or with a non-targeting control siRNA. Cells were then incubated with 150 μ M Oroxylin A for 48 h. Mitochondria were isolated, then deacetylated cyclophilin D and ANT-1 was immunoprecipitated as above, respectively

Back to article page