Figure 1

Ca²⁺-dependent activation of whole-cell currents in normal but not Scott lymphocytes. Original recordings of whole-cell currents measured in normal lymphocytes (a) and lymphocytes from two patients with Scott disease (c and e). Cells were kept under current clamp and were voltage clamped in intervals from −100 to +50 mV in steps of 10 mV. Ionomycin (1 μM) activated a whole-cell current only in normal lymphocytes (a). Current/voltage relationships were obtained in normal (b) and Scott lymphocytes (d and f). Note the activation of a large whole-cell current by ionomycin (filled circles) in normal but not in Scott lymphocytes. (g and h) Activation of a whole-cell Cl⁻ current by ionomycin (1 μM) in normal B lymphocytes, using 145 mM NMDG⁺Cl⁻ in pipette and bath. Note the outward rectification of the whole-cell current and the pronounced inhibition by 20 μM AO1. (i) Inhibition of ionomycin-activated outward currents by replacement of extracellular Cl⁻ with gluconate (5 Cl⁻). (j) Inhibition of ionomycin-induced whole-cell currents (current densities) and depolarization in normal B lymphocytes after knockdown of Ano6 expression with siRNA. Mean±S.E.M. (number of cells). ‘*’ denotes significant activation by ionomycin or effect of siRNA for Ano6 (si-Ano6); P<0.05; paired t-test