Figure 4
Activation Ano6 Cl− currents by activation of Fas receptors. (a) Whole-cell current overlays obtained in normal lymphocytes before and after incubation with FasL (0.5 μg/ml; 2 h), and inhibition by Cl− replacement (5 Cl−) and tannic acid (TA; 20 μM). Cells were voltage clamped from −100 to +50 mV in steps of 10 mV. Arrowhead indicates currents at clamp voltage of 0 mV. (b) Current/voltage relationship of the FasL-activated whole-cell current and effect of TA. (c–e) Calculated whole-cell conductances in normal, ScottUSA, and ScottUK lymphocytes and effects of 5 Cl−, TA and NPPB. Dashed line indicates conductance before incubation with FasL (control). Mean±S.E.M. (number of cells). ‘*’denotes significant inhibition; P<0.05; paired t-test. ‘#’denotes significant activation by FasL; P<0.05; unpaired t-test
