Figure 5
Phospholipid scrambling by ionomycin but not by FasL is impaired in Scott lymphocytes. (a) Percentage of phosphatidylserine (PS)-exposing lymphocytes after 10 min stimulation with 10 μM ionomycin in presence of 1 mM CaCl2 for normal and Scott lymphocytes as measured after labeling with annexin A5 for 5 min. (b) Effect of NPPB (100 μM), TA (50 μM) and replacement of Cl− by gluconate on ionomycin-induced PS exposure in healthy control lymphocytes. (c) Percentage PS-exposing lymphocytes after 4 h incubation with 0.5 μg/ml FasL in presence of 1 mM CaCl2 for control and Scott lymphocytes in presence or absence of 20 μM Q-VD-Oph. (d) No effects of NPPB (100 μM), TA (50 μM) and Cl− replacement on FasL-induced PS exposure in healthy control lymphocytes were observed. (e) Caspase-3 activity of control and Scott lymphocytes after 4 h stimulation with FasL as measured by a caspase activity assay. (f) Percentage of PS-exposing lymphocytes after incubation with 50 μM ABT-737. Mean±S.D. (n=3–12). ‘#’ denotes significant activation of PS exposure or caspase; P<0.05; unpaired t-test
