Table 1 Proteins secreted into cell culture media with significant fold changes following induction of the death-switch after 6, 8 and 24 h

From: A caspase-3 ‘death-switch’ in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers

Protein Name	Fold change at each time-point post dox (log₂)
	6 h	8 h	24 h
Secreted proteins
AGRN Agrin	−1.41	−1.40	−1.92
B2M Beta-2-microglobulin	−1.634	−1.66	−2.50
CST3 Cystatin-C	−1.57	−1.60	−2.34
GRN Granulins	−0.98	−1.07	−2.16
GSN Gelsolin	−1.14	−1.02	−1.74
HSPG2 Basement membrane-specific heparan sulfate proteoglycan core protein	−1.51	−1.52	−2.53
KLK6 Kallikrein-6	−0.90	−1.04	−2.03
LGALS3BP Galectin-3-binding protein	−1.03	−1.12	−1.21
THBS1 Thrombospondin-1	−1.06	−1.03	−1.61
TWSG1 Twisted gastrulation protein homolog 1	−0.99	−0.88	−1.85
SERPINA1 Alpha-1-antitrypsin	−1.43	−1.23	−2.06
LRG1 Leucine-rich alpha-2-glycoprotein	−0.650	1.00	−1.36
LYZ Lysozyme C	−1.44	−1.71	−1.80
SPINT1 Kunitz-type protease inhibitor 1	−0.14	−0.08	−0.58
HP Haptoglobin	0.16	0.20	−0.91
AMY1B Alpha-amylase 1	0.38	0.22	−1.20
MDK Midkine	−1.31	−1.26	−1.89
Membrane proteins
ADAM17 Disintegrin and metalloproteinase domain-containing protein 17	−0.41	−0.83	−1.39
LSR Lipolysis-stimulated lipoprotein receptor	−1.08	−1.08	−1.99
SDC4 Syndecan-4	−0.58	−0.74	−1.36
SPINT2 Kunitz-type protease inhibitor 2	−0.49	−0.39	−0.71
SHISA5 Protein shisa-5	−1.73	−1.71	−2.91
EFNB1 Ephrin-B1	0.33	0.82	−0.07
ST14 Suppressor of tumorigenicity protein 14	0.09	0.31	1.15
GAPDH Glyceraldehyde-3-phosphate dehydrogenase	0.29	−0.06	1.17
*CD44 CD44 antigen	−0.44	0.41	0.82
DSG2 Desmoglein-2	−0.00	0.66	0.48
BCAM basal cell adhesion molecule precursor	0.08	0.64	0.82
Cytoplasmic proteins
CTSH Cathepsin H	−0.72	−1.27	−2.56
HNRNPA2B1 Heterogeneous nuclear ribonucleoprotein 2B1	0.60	0.09	−0.32
TUBA1B Tubulin-α-1B	0.61	0.47	0.26
TMOD2 neuronal tropomodulin	0.66	0.77	0.07
S100A11 Protein S100-A11	0.33	0.07	−0.98
SERBP1 Plasminogen activator inhibitor 1 RNA-binding protein	0.65	0.21	0.18
GAPDH Glyceraldehyde-3-phosphate dehydrogenase	0.29	−0.06	1.17
*HSP90 heat shock 90kDa protein	0.26	0.21	0.64
*NCL Nucleolin	0.10	−0.03	1.16
PFN1 Profilin-1	−0.17	−0.39	0.65
PGK1 Phosphoglycerate kinase 1	0.25	−0.08	0.78
PKM2 Pyruvate kinase isozymes M1/M2	0.13	0.11	0.82
SET Protein SET	0.44	0.33	2.27
Nuclear proteins
HIST1H1B Histone H1.5	0.10	0.12	−1.16
HIST1H1D Histone H1.3	0.21	0.16	−0.98
HIST1H1E Histone H1.4	−0.07	0.02	−0.91
HIST1H2BN Histone H2B type 1-N	0.27	0.23	−1.13
HNRNPA2B1 Heterogeneous nuclear ribonucleoprotein 2B1	0.60	0.09	−0.32
SERBP1 Plasminogen activator inhibitor 1 RNA-binding protein	0.65	0.21	0.18
S100A11 Protein S100-A11	0.33	0.07	−0.98
*HMGB1 High mobility group protein B1	0.66	0.39	0.89
**NCL Nucleolin*	0.10	−0.03	1.16
PKM2 Pyruvate kinase isozymes M1/M2	0.13	0.11	0.82
SET Protein SET	0.44	0.33	2.27
Proteins with unspecified location
ATIC Bifunctional purine biosynthesis protein PURH	0.62	0.56	0.22
SPINK5 Serine peptidase inhibitor	−0.82	−0.69	−1.77
MDH2 Malate dehydrogenase	0.04	−0.06	−0.96
EEF1D Eukaryotic translation elongation factor 1 delta	0.23	−0.02	0.67
TUBB3 Tubulin-β3	0.80	0.61	0.79

Proteins identified in the in vitro proteomic screen with differential expression levels in serum-free media after death-switch induction, grouped by subcellular localization. Fold changes (compared with uninduced cells at 24 h) are expressed as log₂ values. Numbers that represent significant fold changes are in bold lype. Proteins that were verified by alternative methods are in bold type and prefixed by an asterisk (HMGB1, Hsp90, CD44 and Nucleolin). Proteins in the CASBAH database⁷ are highlighted in italics (gelsolin, tubulin α-1-B, Hsp90, nucleolin, SET and eukaryotic elongation initiation factor 1 delta).

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Table 1 Proteins secreted into cell culture media with significant fold changes following induction of the death-switch after 6, 8 and 24 h

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