Figure 1

TAT-IDPS-induced apoptosis in DL-BCL cell lines. (a) Representative dot plots from flow cytometry analysis of apoptosis using annexin V-FITC/PI-stained KARPAS422, OCI-LY-1, SU-DHL-4, TOLEDO, and PFEIFFER, either untreated or treated with 10 μM TAT-IDPS for 24 h or with 1 μM staurosporine for 6 h (10 000 cells per analysis). The apoptotic population was identified as the annexin V-FITC-positive fraction (Q2+Q4). (b, c) Quantitative analysis of four independent experiments of the (b) TAT-IDPS- and (c) staurosporine-induced apoptosis (Δ apoptotic fraction=apoptotic population in treated cells−apoptotic population in untreated cells). Data are expressed as the average ±S.D.