Figure 3 | Cell Death & Disease

Figure 3

From: IP3R2 levels dictate the apoptotic sensitivity of diffuse large B-cell lymphoma cells to an IP3R-derived peptide targeting the BH4 domain of Bcl-2

Figure 3

TAT-IDPS triggers IP3R-mediated [Ca2+] rises in SU-DHL-4 but not in OCI-LY-1. (a) Representative traces from fluorimetric analysis of the TAT-IDPS-induced Ca2+ responses in SU-DHL-4 and OCI-LY-1 using the ratiometric Ca2+ indicator Fura2-AM. Vehicle solution and TAT-Ctrl were used as negative controls. The ratio of emitted fluorescence of Fura2 (F340/F380) was monitored and the three different treatments were added 60 s (second arrow) after the addition of 1 mM EGTA (first arrow). (b) Representative traces of Ca2+ responses induced by the treatment with 10 μM TAT-IDPS (second arrow) in SU-DHL-4 (left panel) and OCI-LY-1 (right panel) pretreated without (black traces) or with (gray traces) 10 μM XeB for 30 min in the presence of 1 mM EGTA (first arrow). TAT-Ctrl was used as negative control (light gray traces). Recordings without XeB were exposed to the vehicle (dimethyl sulfoxide). (c) Analysis of the 10 μM thapsigargin (TG)-induced Ca2+ responses in SU-DHL-4 and OCI-LY-1 pretreated without or with 10 μM TAT-Ctrl or TAT-IDPS for 30 min. The curves are representative of three independent experiments

Back to article page