Figure 1
Effect of cannabinoids on key metabolic proteins. Western blot analyses of (a) phospho-AMPK, (b) phospho-p70S6K, (c) HIF-1 alpha, and (d) pyruvate dehydrogenase kinase (PDHK) were performed after treatment of Panc1 cells for 1, 12, and 24 h with 200 μM GW or ACPA. The bands were scanned as digital peaks and the areas of the peaks were calculated in arbitrary units, as described in Materials and Methods. The value of α-tubulin was used as a normalizing factor and quantifications represent the ratio phosphorylated/total protein. Quantification values are the means of three independent experiments (±S.D.). Statistical analysis: *P<0.05, **P<0.01, and ***P<0.001
