Figure 3
Effect of cannabinoids on glycolysis. Panc1 were treated for 12 h with (a) GW or (b) ACPA and the metabolites analysis was performed as reported in Materials and Methods.FDP, fructose 1,6-bisphosphate; F6P, fructose-6-phosphate; G3P, glyceraldehyde-3-phosphate; G6P, glucose-6-phosphate; LACT, lactate; PEP, phosphoenolpyruvate. (c) Glucose consumption was measured as reported in Materials and Methods. Values are the means of three independent experiments (±S.D.). (d) PKM2 activity was performed after treatment of Panc1 cells for 12 or 24 h with 200 μM GW or ACPA, as reported in Materials and Methods. Values are the means of three independent experiments (±S.D.). Statistical analysis: *P<0.05, **P<0.01, and ***P<0.001
