Figure 3 | Cell Death & Disease

Figure 3

From: Loss of connective tissue growth factor as an unfavorable prognosis factor activates miR-18b by PI3K/AKT/C-Jun and C-Myc and promotes cell growth in nasopharyngeal carcinoma

Figure 3

CTGF inhibited the expression of miR-18b, and miR-18b directly targets CTGF in NPC. (a) MiR-18b was notably upregulated in SiRNA-CTGF-treated 6-10B NPC cells by miRNA arrays analysis. (b) Real-time PCR confirmed the reliability of miRNA arrays in SiRNA-CTGF-treated HONE1, 6-10B and shRNA-6-10B-treated cells. (c) CTGF expression was repressed or elevated with the transfection of miR-18b mimics and inhibitor, respectively, at 100 nm in NPC 6-10B cells. (d) Luciferase reporter assay was used to determine whether miR-18b could directly target the 3′UTR of CTGF in NPC cells. Lanes 1 and 2: a significant decrease of luciferase activity was indicated in miR group. Lanes 5 and 6: the activity of mt 3′UTR vector was unaffected by a simultaneous transfection with miR-18b. Lanes 3 and 4: co-transfection with miR-18b inhibitor and wt 3′UTR vector led to a marked increase of luciferase activity. Data are presented as mean±S.D. for three independent experiments (*P<0.05)

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