Figure 4 | Cell Death & Disease

Figure 4

From: Loss of connective tissue growth factor as an unfavorable prognosis factor activates miR-18b by PI3K/AKT/C-Jun and C-Myc and promotes cell growth in nasopharyngeal carcinoma

Figure 4

C-Jun and C-Myc directly positively modulated miR-18b expression in NPC. (a) siRNA (fragment 1) was used to suppress the expression of C-Jun in NPC 6-10B cells. (b) The expression of C-Myc was suppressed by its specific siRNA (fragment 2) in NPC 6-10B cells. (c) Knocking down C-Jun downregulated the expression of miR-18b in NPC 6-10B cells. (d) Reduced C-Myc expression downregulated the expression of miR-18b in NPC 6-10B cells. (e and f) Crosslinked chromatin preparation from mock and pcDNA3.1-C-Myc or pcDNA3.1-C-Jun-transfected 6-10B cells was immunoprecipitated with anti-C-Myc, anti-C-Jun, or a normal rabbit IgG. The AP1 binding sites on the immunoprecipitated DNA was determined by quantitative RT-PCR. Amplification of input chromatin (input) before immunoprecipitation was served as positive controls for chromatin extraction and PCR amplification. Chromatin immunoprecipitation using a nonspecific antibody (normal human IgG) served as negative controls. QPCR analysis indicated that C-Jun and C-Myc could bind more miR-18b promoter region than that in control group in NPC 6-10B cells (*P<0.05)

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