Figure 1
1H-NMR analyses of different metabolic profiles in HIPK2-proficient (HIPK2+/+) compared with HIPK2-depleted (siHIPK2) cancer cells. (a) Representative 1H-NMR spectra (400 MHz) of aqueous extract from HIPK2+/+ and siHIPK2 cancer cells. Peak assignments: myo-ino; tau (taurine); tCho (total choline-containing metabolites) resonance including choline (Cho), phosphocholine (PCho); glycerophosphocholine (GPCho); tCr (total creatine: creatine plus phosphocreatine); GS (glutathione); Glt (glutamate); Glx (glutamate+glutamine+glutathione); Acetate; alanine (Ala); and lactate (Lac). p.p.m., parts per million. (b) Score plot of the first two principal components (PC1 and PC2) in HIPK2+/+ (dotted circle) and siHIPK2 (dashed circle) cells. Level of confident=80%. (c) 2D loading plot showing the critical metabolites for different segregation between the two cell lines. In particular, PCho, 3.22 p.p.m.; myo-ino, 4.05 p.p.m.; Lac, 1.33 p.p.m.; and tCr, 3.04 p.p.m. were responsible for segregation in two separate cluster in PC1 versus PC2 score plot. (d) Intracellular concentrations (nmol/106 cells) of 1H-NMR detectable metabolites in HIPK2+/+ and siHIPK2 cells. Significant increases in: Lac, P=0.015; Ala, P=0.007; glycine (gly, P=0.006; tCho, P=0.002; Glt, P=0.004; and glutamine, P=0.007. Significant decrease intCr levels in siHIPK2 compared with HIPK2+/+ cells (P=0.007)
