Figure 5 | Cell Death & Disease

Figure 5

From: Glucose restriction induces cell death in parental but not in homeodomain-interacting protein kinase 2-depleted RKO colon cancer cells: molecular mechanisms and implications for tumor therapy

Figure 5

Zinc supplementation induces cell death in glucose-starving siHIPK2 cells. (a) Lactate production in medium of HIPK2+/+ cells cultured for 16 h in glucose-free medium (glu stv) alone or in combination with ZnCl2 (100 μM). Results are the mean of two independent experiments performed in quadruplicate, ±S.D. *, the difference was significant at P<0.05. (b) siHIPK2 cells were cultured in glucose-free medium (glu stv) or treated with 12 mM 2-DG alone for 48 h or in combination with ZnCl2 (100 μM), before trypan blue staining was performed. The result is the mean of three independent experiments performed in duplicate ±S.D. *. P<0.05. (c) Cytofluorimetric dot plot analysis of the Annexin V-FITC versus propidium iodide (PI) staining of siHIPK2 cells cultured with glucose-free medium (glu stv) for 48 h alone or in combination with ZnCl2 (100 μM). A representative experiment out of three performed with similar results is shown. Annexin V+/PI− and Annexin V+/PI+ were considered early apoptotic and late apoptotic or necrotic cells, respectively. (d) Percentage of Annexin V+/PI− and Annexin V+/PI+ cells was calculated by cytofluorimetric analysis shown in (c) using CellQuest Software

Back to article page