Figure 6 | Cell Death & Disease

Figure 6

From: Glucose restriction induces cell death in parental but not in homeodomain-interacting protein kinase 2-depleted RKO colon cancer cells: molecular mechanisms and implications for tumor therapy

Figure 6

Inhibition of autophagy increased cell death. (a) Equal amount of total cell extracts from HIPK2+/+ and siHIPK2 cells left untreated or treated for 24 h with glucose-free medium (glu stv) alone or in combination with autophagy inhibitors 3-MA (5 mM) or CQ (25 μM) were assayed by western immunoblotting of LC3-I/II protein level. β-actin was used as protein loading control. A representative result of three independent experiments is shown. (b) HIPK2+/+ and siHIPK2 cells were cultured for 24 h in glucose-free (glu stv) medium alone or in combination with CQ (25 μM), before trypan blue staining was performed. The result is the mean of three independent experiments performed in duplicate ±S.D. *P=0.001. (c) siHIPK2 cells were cultured in glucose-free medium (glu stv) alone for 48 h or in combination with ZnCl2 (100 μM) and CQ (25 μM) before cell death analysis was performed by Annexin V/PI staining and evaluated by immunocytochemistry. Two hundred cells were counted in duplicate in two independent experiments and plotted as percentage compared with control, ±S.D

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