Figure 7 | Cell Death & Disease

Figure 7

From: Glucose restriction induces cell death in parental but not in homeodomain-interacting protein kinase 2-depleted RKO colon cancer cells: molecular mechanisms and implications for tumor therapy

Figure 7

Akt phosphorylation is inhibited by CQ and zinc treatments. (a) Equal amount of total cell extracts from siHIPK2 cells left untreated or treated for 24 h with glucose-free medium (glu stv) alone or in combination with autophagy inhibitors 3-MA (5 mM) or CQ (25 μM) were assayed by western immunoblotting of JNK phosphorylation and total JNK levels. A sample of HIPK2+/+ cells treated with glucose restriction for 24 h is included as positive control (ctr+). (b) Equal amount of total cell extracts from siHIPK2 cells left untreated or treated for 24 h with glucose-free medium (glu stv) alone or in combination with CQ (25 μM) or ZnCl2 (100 μM) were assayed by western immunoblotting of Akt phosphorylation and total Akt levels. β-actin is used as protein loading control. A representative result out of two independent experiments is shown

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