Figure 1

Generation of IL-32β transgenic mice. (a) Scheme for IL-32β transgenic generation. (b) PCR analysis (genotyping) was performed to analyze the existence of IL-32β gene in transgenic mice, as described in Materials and Methods. (c) RT-PCR and western blott analyses for IL-32β in the tissues of IL-32β transgenic and nontransgenic mice. (d) Detection of IL-32 in the sera of transgenic or nontransgenic mice. The results are expressed as mean±SD of three mice. * Significant difference from nontransgenic mice (*P<0.05)