Figure 4
From: Gossypol and an HMT G9a inhibitor act in synergy to induce cell death in pancreatic cancer cells
BNIP3 mRNA and protein levels are increased upon compound treatment. (a) Fold change in gene expression in PANC-1 cells treated for 3 days with 2.5 μM gossypol alone or the combination of 2.5 μM gossypol and 2.5 μM BRD4770. The 10 gene transcripts that showed the greatest fold induction between the two conditions are shown. The total RNA was extracted, and the 84 key genes involved in autophagy were profiled by a 384-well format human autophagy pathway-focused expression real-time PCR array. Six housekeeping genes were used as internal controls for data normalization (see the Materials and Methods section). Data for BRD4770 treatment were generated by gene expression profiling (see the Materials and Methods section). (b) Protein levels of BNIP3, BNIP3L, beclin1, and BCL-xL were assessed in PANC-1 cells treated for 3 days with the indicated concentrations of BRD4770. BNIP3 was observed as a 30-kDa monomer and a 60-kDa dimer. Tubulin was used as an internal loading control
