Figure 1 | Cell Death & Disease

Figure 1

From: Alterations in c-Myc phenotypes resulting from dynamin-related protein 1 (Drp1)-mediated mitochondrial fission

Figure 1

Characterization of cell lines and mitochondria. (a) Stable expression of Drp1. Rat1a MycER cells were stably transduced with a lentiviral vector encoding a full-length, rat Drp1-V5 fusion protein (Drp1 cells) or with the empty parental vector (Vector cells). Drp1 protein expression was assessed by immunoblotting for V5 epitope expression. (b) Quantification of mitochondrial mass. Cell lines were evaluated by flow cytometry after staining with MitoTracker Green or NAO to quantify overall mitochondrial mass. Drp1:Vector mean fluorescence ratios are expressed above each flow histogram. The results shown are typical of at least 4–6 independent experiments performed at different times. (c) Representative confocal images obtained on cells stably transfected with a plasmid encoding DsRED2-Mito. Mean fluorescence intensities were also quantified by flow cytometry with the Drp1:Vector ratio being shown beneath the panel. See Supplementary Video S1 for additional images

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