Figure 3
Aberrant ETC complexes in Drp1 cells. (a) BNGE of purified mitochondria from Vector and Drp1 cells. Each of the major complexes and supercomplexes (SC) is indicated. (b) Quantification of ETC differences in Vector and Drp1 cells. Complex abundance was determined by scanning densitometry of Coomassie blue-stained blue-native gels similar to those depicted in panel a and arbitrarily expressed relative to Complex IV, which showed no change between the two cell lines. An aberrant complex (Complex ‘X’), previously seen in myc−/− cells4 was also observed at a significantly elevated level in Drp1 cells
