Figure 8
AICAR restores ATP levels in Drp1 cells and accelerates Myc-dependent apoptosis. (a) Drp1 cells were seeded in 96-well plates as described for Figure 5b. The following day, fresh medium containing 250 μ M AICAR or vehicle alone was added and ATP levels were determined 4 h later. The histogram shows the mean relative ATP levels±1 S.E. from quadruplicate samples. (b) Parallel cultures of cells, maintained in 100-mm plates, were treated as described in panel (a) and were evaluated by immunoblotting for total and pAMPK levels. In Drp1 cells, the levels of pAMPK relative to total AMPK levels were 2.5-fold higher compared with Vector cells. (c) Drp1 cells were maintained under standard growth conditions (Figure 6a) for 3 days in the presence or absence of 4-HT and 250 μ M AICAR. The total number of viable cells was then determined. The results are expressed as the mean of triplicate sets of cultures±1 S.E. (d) Changes in the mean diameter of Drp1 cells following a 3-day exposure to AICAR. Cells were maintained and counted as described in Figure 6d. The results represent the mean diameters±1 S.E. of at least 3000 cells from triplicate cultures
