Figure 6 | Cell Death & Disease

Figure 6

From: The embryonic transcription factor Brachyury blocks cell cycle progression and mediates tumor resistance to conventional antitumor therapies

Figure 6

Brachyury modulates expression of cell cycle regulatory proteins. Western blot analysis of Brachyury and the cell cycle regulatory proteins Rb, cyclin D1 and p21 was performed using (a) the A549 tumor cell pair at indicated times after release from cell cycle arrest by serum starvation or (b) asynchronous cultures of H460 control versus Brachyury shRNA-1 and -2 cells. (c) Two tumor cell lines derived from single-cell cloning of H460 cells were analyzed for expression of Brachyury in relation to Rb and p21. (d) H460 cells transfected with p21 expression vector or pCMV control were analyzed for p21 and Rb expression by western blot and (e) growth kinetics over a 5-day period. (f) Indicated cells were treated with cytotoxic therapies and assayed for survival in comparison with untreated cells. (g) The H460 cell pair transfected with a pool of nonspecific control siRNA or p21-specific siRNAs was treated with γ-radiation (1 Gy) and evaluated for cell death by using CellTiter-Glo (Promega). (**P<0.01; ***P<0.001)

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