Figure 3
From: A novel radioprotective function for the mitochondrial tumor suppressor protein Fus1
Proliferation and aberrant mitosis in Fus1+/+ and Fus1−/−intestinal crypts after 12 Gy WBI. (a) Sections of distal jejunum from BrdU-injected sham-irradiated and irradiated WT and Fus1 KO mice at different time points after irradiation were prepared and stained with anti-BrdU. Shown is a bar graphs of the average numbers of BrdU-positive proliferating cells per crypt in each treatment group with S.E.M. from at least three mice in each group, 100 half-crypt sections per mouse. (b and c) Sections of distal jejunum from sham-irradiated and irradiated WT and Fus1 KO mice at 24 h post IR were prepared and stained with H&E. Shown is a bar graphs of the average numbers of cells with normal and aberrant mitoses per crypt in each treatment group with S.E.M. from at least three mice in each group, 100 half-crypt sections per mouse (b). Shown are micrographs of half-crypts with aberrant mitoses from Fus1−/−jejunum at 24 h post IR indicated by red arrows ( × 400 magnification) (c)
