Figure 2 | Cell Death & Disease

Figure 2

From: Pleiotropic actions of miR-21 highlight the critical role of deregulated stromal microRNAs during colorectal cancer progression

Figure 2

Fluorescence from MRC5 fibroblasts stably transfected with miR-21/GFP or control miR-SCC/GFP co-expression plasmids highlight the morphological changes associated with ectopic miR-21 expression in fibroblasts ( × 40 magnification). Western blot analysis reveals that MRC5 fibroblasts in which miR-21 has been stably induced express upregulated α-SMA compared with controls. (b) Immunofluorescence staining for α-SMA in MRC5 fibroblasts stably expressing either miR-21 or miR-SCC at × 40 magnification: MiR-21 but not miR-SCC is associated with α-SMA stress fibres formation, following treatment with low doses of TGF-β (2 ng/ml) or culture medium extracted from SW480 CRC cells (CMSW480). High doses of TGF-β (10 ng/ml) also induce stress fibre deposition in miR-SCC-transfected cells, which serves as a positive control (*P<0.05; **P<0.005)

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