Figure 1
From: Salinomycin induces cell death via inactivation of Stat3 and downregulation of Skp2
Multidrug resistance and MDR-1 expression in ovarian cancer cell lines, including OVCAR-8, NCI/ADR-RES, and DXR cells. OVCAR-8, NCI/ADR-RES, and DXR cells were treated with the indicated concentrations of (a) doxorubicin, (b) paclitaxel, (c) etoposide, and (d) cisplatin for 72 h, and cell viability was determined by MTS assay. Data are presented as averages of triplicate measurements, with error bars representing standard deviations. *P<0.05, **P<0.005, and ***P<0.0005. (e) The levels of MDR-1 mRNA were determined by RT-PCR in each cell line. (f) Equal amounts of cell lysates were subjected to immunoblot analysis using MDR-1 antibody. β-Actin was used as a loading control. (g) Cells were incubated with 10 μM rhodamine123 or 50 μM doxorubicin for 2 h, and the accumulation of rhodamine123 (left panel) and doxorubicin (right panel) was measured by flow cytometric analysis. These experiments were performed twice on separate samples with comparable results
