Figure 2
From: miR-874 regulates myocardial necrosis by targeting caspase-8
miR-874 is able to regulate necrosis in the heart. (a) miR-874 levels during myocardial I/R. Mice were induced to undergo cardiac I/R. Area at risk and the remote area were prepared at the indicated time for qRT-PCR analysis of miR-874 levels, n=6, *P<0.05 versus 0 min or sham. (b and c) Knockdown of miR-874 attenuates myocardial necrosis upon I/R. Mice were subjected to I/R as described in methods. b: Immunohistochemistry for myosin antibody incorporation into the heart (green); red is a wheat germ stain for cell membranes; nuclei are shown in blue. Percentage of cells with myosin antibody infiltration was shown (c), n=6, *P<0.05 versus I/R alone. (d) Knockdown of miR-874 inhibits MI. Mice were treated as described in b. Infarct sizes were shown, n=6, *P<0.05 versus I/R +NC. (e) Knockdown of miR-874 preserves cardiac function upon I/R. Transthoracic echocardiographic analysis was performed after 60 min ischemia followed by 1 week reperfusion. Diastolic left ventricular internal diameters (LVIDd); Systolic left ventricular internal diameters (LVIDs); fractional shortening of left ventricular diameter (FS), *P<0.05 versus I/R alone
