Figure 1 | Cell Death & Disease

Figure 1

From: ifn-γ-dependent secretion of IL-10 from Th1 cells and microglia/macrophages contributes to functional recovery after spinal cord injury

Figure 1

Improved recovery induced by Th1 cells after SCI depends on IFN-γ. Enhanced recovery from SCI by Th1 transfer is dependent on interferon-γ (ifn-γ) in transferred Th1 cells. (a) Level of IFN-γ secreted from Th1 cells, Th1 ifn-γ−/− cells, and Th17 cells, which was detected by ELISA(n=3). (b) Comparison of T-bet production in helper T cells differentiated ex vivo, which was assessed by flow cytometry histogram. Representative data from three independent experiments. (c) Time course of hindlimb locomotion using the BMS with adoptive transfer of 1.0 × 107 each helper T-cell subsets (PBS, n=7; Th1 n=7; Th1 ifn-γ−/−, n=5). (d) Time course of the grid-walk test (PBS, n=4; Th1, n=6; Th1 ifn-γ−/−, n=4). Data are presented as mean±S.E.M. *P<0.05, ***P<0.001 versus the PBS group, #P<0.05 versus Th1 group (two-way ANOVA with repeated measures, Bonferroni post-test)

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