Figure 3 | Cell Death & Disease

Figure 3

From: ifn-γ-dependent secretion of IL-10 from Th1 cells and microglia/macrophages contributes to functional recovery after spinal cord injury

Figure 3

Neutralization of IL-10 in the CNS attenuates functional recovery by Th1 cells transfer. IL-10 expressed in the CNS partially contributes to functional recovery after SCI with adoptive transfer of Th1-conditioned cells. (a) Upper histograms: comparison of IL-10 production from MG/MΦs evaluated using histograms of intracellular cytokine staining. The histogram is representative of four independent experiments. All histograms were gated from the populations of MG or MΦs as shown in Figure 2c. Lower graph: quantification of IL-10 production as indicated above. *P<0.05 versus the PBS group, #P<0.05 versus Th1 group (one-way ANOVA, Bonferroni post-test). (b–d) Functional recovery from SCI evaluated by: BMS scores (b), grid-walk test (c), and inclined-plane test (d) in Th1-conditioned cell-transferred SCI mice treated with control IgG or anti-IL-10 antibody into the cerebral ventricles (i.c.v.; control rat IgG+Th1, n=15; anti-IL-10+Th1, n=13 (BMS scores and inclined-plane test)/control IgG+Th1, n=9; anti-IL-10+Th1, n=8 (grid-walk test)). *P<0.05, **P<0.01 versus control IgG+Th1 group (two-way ANOVA with repeated measures, Bonferroni post-test)

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