Figure 1 | Cell Death & Disease

Figure 1

From: DAX-1, as an androgen-target gene, inhibits aromatase expression: a novel mechanism blocking estrogen-dependent breast cancer cell proliferation

Figure 1

Long-term Mb administration inhibits MCF-7 cell proliferation and induces apoptosis. (A) Cell counting by trypan blue exclusion test in cells treated as indicated (6 days). (B) Cells treated with 10−8 M Mb for 6 days were subjected to TUNEL nuclear staining (a, b) and viewed by a fluorescent microscope. DAPI staining for nuclei detection (c, d); bright field (e, f). Apoptotic cells were photographed at × 10 magnification and then counted using Image J software. (C) Histograms represent the apoptotic index % apoptotic cells/total cell number in the field). *P<0.01

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