Figure 6 | Cell Death & Disease

Figure 6

From: DAX-1, as an androgen-target gene, inhibits aromatase expression: a novel mechanism blocking estrogen-dependent breast cancer cell proliferation

Figure 6

DAX-1 overexpression decreases proliferation and induces apoptosis in MCF-7 cells. (A) Cells were transfected with control plasmid (pcDNA3) or a human DAX-1 expressing plasmid (hDAX-1). Transfection was renewed every 2 days and cells were counted on day 6 using trypan blue exclusion test. *P<0.05. (B) Immunoblotting analysis for PARP cleavage, Caspase 9, Bcl-2, Bad and DAX-1 expression in total extracts from cells transfected as above. β-Actin, loading control. (C) Histograms represent the ratio between Bad and bcl-2 band intensities (from B) evaluated as optical density arbitrary units and expressed as fold induction over control. *P<0.01. (D) MCF-7 cells, transfected as above, were subjected to TUNEL nuclear staining (a, b) and viewed by a fluorescent microscope. DAPI staining for nuclei detection (c, d); bright field (e, f). Apoptotic cells were photographed at × 10 magnification and then counted using Image J software. (E) Histograms represent the apoptotic index (% apoptotic cells/total cell number in the field). *P<0.01

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