Figure 5
From: H-Ras transfers from B to T cells via tunneling nanotubes
Transferred GFP-H-RasG12V-enriched PM patches maintain proper in–out orientation. B721.221 cells expressing GFP-H-RasG12V and Jurkat cells labeled with CellVue Claret Far Red Fluorescent dye were seeded as detailed above. Two hours post seeding, cells were fixed and stained with mAbs that specifically recognize the extracellular domain of CD86, followed by staining with Alexa Fluor 555-conjugated secondary antibodies. Panels (single colored and merged) depict a single confocal section of a typical Jurkat cell decorated with a GFP-H-RasG12V patch (scale bars represent 5 μm)
