Figure 1
From: The role of thioredoxin 1 in the mycophenolic acid-induced apoptosis of insulin-producing cells
Changes in Trx1 gene and protein expression levels in INS-1E cells and isolated rat islets after MPA treatment. (a, b) INS-1E cells were treated with 10 μM MPA for 12h, 24h, or 36 h or co-treated with 10 μM MPA plus 100 μM GTP for 36 h. (c, d) Islets isolated from a rat were treated with 40 μM MPA for 12h, 24h, or 36 h or co-treated with 10 μM MPA plus 100 μM GTP for 36 h. (a) To quantify Trx1 gene expression in INS-1E cells, mRNA levels were assessed by qPCR. Trx1 gene expression levels gradually diminished after MPA treatment, but this effect was inhibited by GTP co-treatment. Results are the means±SEMs of three independent experiments. ***P<0.0001 versus the untreated control (CTL). GAPDH was used as an internal control to normalize gene expressions. (b) Cell lysates (40 μg) were loaded onto gels and immunoblotted. In INS-1E cells, Trx1 protein levels were progressively reduced by MPA treatment. β-Actin was used to confirm equal loading. (c) The mRNA expressions of Trx1 in islets were evaluated by qPCR. Trx1 gene expression decreased progressively after MPA treatment. ***P<0.0001 versus CTL. (d) Cell lysates (40 μg) were loaded onto gels and immunoblotted. In isolated islets, Trx1 protein levels were progressively reduced by MPA treatment
