Figure 3
From: The role of thioredoxin 1 in the mycophenolic acid-induced apoptosis of insulin-producing cells
The effects of Trx1 gene knockdown and Trx1 overexpression in INS-1E cells. (a) INS-1E cells were either mock transfected (Mock) or transfected with Trx1 siRNA (siTrx1) or full-length Trx1 cDNA (Trx1 O/E). Results are presented as means±SEMs of three independent experiments performed in duplicate. **P<0.01; ***P<0.001 compared with mock-transfected cells (Mock). (b) Cells were treated with 10 μM MPA for the indicated times, and cell viabilities were measured using an MTT assay. The survival rate of Trx1-overexpressing INS-1E cells treated with MPA was greater than that of cells treated with MPA. However, the survival rate of Trx1 knockdown cells was poorer than that of MPA-treated cells. **P<0.01 versus MPA-treated cells at 12 h, ***P<0.001 versus MPA-treated cells at 24 h and 36 h (n=4)
