Figure 4

Expression levels of p-MKK4/7, p-JNK, and cleaved caspase-3 following MPA treatment. INS-1E cells and INS-1E cells overexpressing Trx1 were treated with 10 μM MPA for 12, 24, or 36 h. Total protein lysates were western blotted. Bar graphs represent quantitative differences in expressions of p-MKK4/7, p-JNK, and cleaved caspase-3. Results are means±SEMs (n=3). **P<0.01, ***P<0.001 versus CTL. (a) P-MKK4/7 expression was higher in INS-1E cells than in cells overexpressing Trx1. Whereas p-MKK4/7 was expressed in INS-1E cells, it was not expressed in cells overexpressing Trx1. (b) P-JNK levels were higher at 24 h and 36 h in INS-1E cells than in cells overexpressing Trx1, and P-JNK was only expressed slightly at 36 h in Trx1-overexpressing cells. (c) Increases in cleaved caspase-3 levels in Trx1-overexpressing cells were lower than in INS-1E cells. Equal loading was confirmed using JNK and β-actin