Figure 1 | Cell Death & Disease

Figure 1

From: Development of a human three-dimensional organotypic skin-melanoma spheroid model for in vitro drug testing

Figure 1

Generation of 3D organotypic skin equivalents. Paraffin sections of skin equivalents (a) compared with normal human skin (b) were H&E stained and immunohistochemically analyzed for expression of keratins 14 and 10, involucrin, filaggrin and laminin 5. (c) Melanoma cells of different tumor progression stages, SBCL2 (RGP), WM115 (VGP) and 451-LU (MM), were seeded on top of the dermal layer before addition of keratinocytes. At day 17 of epidermal differentiation, the localization of melanoma cells within the reconstituted skin equivalents was visualized by H&E staining of paraffin sections. Images show one representative out of three independently performed experiments

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